A new precise, reproducible and validated RP-HPLC method was developed for the simultaneous estimation of Rosuvastatin calcium and Clopidogrel bisulphate in pharmaceutical dosage form. The chromatographic conditions used for the separation was Zodiac C18 (4.6x250mm, 5μ) mobile phase comprised of Methanol: acetonitrile: water (76:18:06 %v/v). The flow rate was 1.0 ml/min with detection at 241 nm. The retention time of Rosuvastatin calcium and Clopidogrel bisulphate was found to be 4.61 and 9.09 min respectively. The linearity was found to be in the range of 10-50 µg/ml for Rosuvastatin calcium and for Clopidogrel bisulphate 45-450 µg/ml with correlation coefficients of 0.9998 and 0.9994 respectively. The proposed method is accurate with 99.6 – 102.40 % recovery for Rosuvastatin calcium and 98.70-100.10 % recovery for Clopidogerl bisulphate and precise (%RSD of repeatability, intraday and inter day variations were 0.43, 0.98 -1.39, 0.27-0.35 for Rosuvastatin calcium and 0.23, 0.36-0.51, 0.39-0.58 for Clopidogrel bisulphate). The limit of detection (LOD) and limit of quantification (LOQ) for Rosuvastatin Calcium 0.2322, 2.9246 µg/ml and for Clopidogerl Bisulphate 0.7037, 9.044 µg/ml respectively. The method can be used for the estimation of dosage form in routine analysis.
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